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#10x chromium single cell immune profiling software

It’s the leading microfluidics platform.

Combining products allows for obtaining single-cell data with multiple dimensions. This results in a low cost per cell in the case of high-throughput projects.ġ0x Genomics offers multiple products, of which some can be combined. The 10x Genomics systems allows for targeting of thousands of cells per sample.

After sequencing, reads are mapped back to the corresponding single cell.

Barcoded fragments are pooled for downstream reactions to create sequencing libraries.

Within the GEM, a reaction takes place where GEL beads are dissolved and molecules from a single cell are captured and barcoded.

Within the Chromium controller, barcoded Gel Beads are mixed with cells or nuclei, enzymes, and partitioning oil to form “GEMs” (Gel Bead-in-emulsion), which are single-cell emulsion droplets.

A pool of Gel Beads, coated with barcoded primers, cells and enzymes are loaded on the 10x Genomics microfluidics chip and placed in the chromium controller.

This how the Next GEM technology in the Chromium controller works: Currently, we offer Single Cell Gene Expression and Single Cell Immune Profiling. The biology behind 10x Genomicsġ0x Genomics offers multiple products that can be used on the chromium controller, each with their own applications. The technique makes use of the Chromium system, a device that enables single-cell sequencing with their Next GEM technology.

Support Sample requirements, shipping, and moreġ0x Genomics is a microfluidics-based method of single-cell RNA sequencing.

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Research Areas Find out how single-cell sequencing is advancing disease understanding and drug development in your research area of interest.Applications Overview From fundamental biology to translational research and drug development, single-cell sequencing applications are revolutionizing science.Research & Development Our expert team drives innovation and versatility.Bulk RNA sequencing Detect transcriptome profiles in populations.VASA-seq Full-length total-RNA sequencing.10x Genomics High-throughput single-cell solutions.SORT-seq Plate-based single-cell transcriptomics.Services Overview Explore multiple single-cell, spatial and bulk RNA sequencing platforms to best suit your biological question.

#10x chromium single cell immune profiling software

From translational immunology and immuno-oncology, to infectious disease research-these technological advancements, along with our intuitive software analysis and visualization tools, will accelerate the understanding of the adaptive immune system.A comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.The Chromium Single cell Immune Profiling Solution Encapsulate up to 80,000 cells in less than 10 minutes.More efficient than leading academic droplet systems.Complete practical solution for single cell analysis Identify rare cell types in heterogeneous populations.High-throughput single cell transcriptomic measurements enable discovery of gene expression dynamics for profiling individual cell types.Key advantages of the Chromium Single cell RNA-Seq Finally, the Chromium™ Software Suite is utilized for processing, analysis and visualization of single cell gene expression data. High-quality next generation sequencing libraries are finished in single bulk reaction. Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells are performed inside each GEM. Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead in emulsion) using the GemCode Technology. The Chromium™ Single Cell RNA sequencing Solution The GemCode™ Technology from 10X Genomics, which combines microfluidics with molecular barcoding and custom bioinformatics software enable analyzing the transcriptomes of thousands of cells at single cell resolution. Advances in single cell RNA quantification techniques have enabled comprehensive study of cell subpopulations within a heterogeneous population.